How To Use Your Liquid Culture Jar: The LC Inoculation Guide

How To Use Your Liquid Culture Jar: The LC Inoculation Guide

  1. Unbox your liquid culture jar kit and remove any packaging.

  2. Set up a clean workspace in a small room with minimal airflow.

  3. Wash your hands and clean your spore or liquid culture syringe (sold separately) with an isopropyl wipe.

  4. Remove the cap from your syringe and connect the needle.

  5. Use the wipe that came with your kit to clean the injection port on the lid.

  6. Remove the cover from your needle and insert the syringe into the injection port.

  7. Add 1ml-10ml of liquid (depending on preference) from your syringe into the jar.

  8. Place the jar in a room temperature space out of direct sunlight and wait for it to grow.

  9. After a week, shake the jar daily to break up the mycelium and speed up growth – do not remove the lid.

  10.  Once 50% of the jar contains mycelium, you’re ready to harvest the liquid culture!

  11. Setup a clean workspace again and wash your hands.

  12. Prepare empty syringes by connecting each one with a needle.

  13. Remove the cover from the needle, insert the syringe into the injection port and pull liquid in.

  14. Put the cover back on each needle and repeat step 13 as necessary. 

  15. Store the liquid culture jar and syringes in the fridge until you’re ready to use them.

  16. Use your new liquid culture syringes to grow mushrooms!

 

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